1、IGV的網址:http://software.broadinstitute.org/software/igv/(java環境)
常見的幾種輸入格式bam/sam(比對文件) TDF(bam的精簡版) bed(注釋文件) gtf/gff(注釋文件)
PSL(blat比對結果) VCF(snp,indel的信息) WIG(UCSC數據庫推薦格式wiggle track format) IGV(IGV默認的格式)
2、操作指南:http://www.docin.com/p-1847147664.html
1、導入參考基因組: genomes >> load genome from file >> reference.fasta
2、導入注釋文件: file >> load from file >> reference.gtf #文件要按坐標排序,已經排過
3、bam格式轉TDF格式: rools >> runigvtools >> count >> sampleA.bam >> run ,再生成另外一個sampleB.bam文件的TDF
bam文件必須和參考基因組文件同一目錄下,否則失敗
4、導入TDF文件: file >> load from file >>sampleA.bam.tdf ;file >> load from file >>sampleB.bam.tdf
5、導入甲基化文件(IGV格式):file >> load from file >> sampleA.igv ;file >> load from file >> sampleB.igv