对样品OUT抽平是为了让各样品的序列数保持一致,便于在同一标准上对各样品进行Alpha多样性分析等,保证有可比性。
Diversity parameters were estimated from OTU and functional gene matrices that were rarefied to an equal number per sample to reduce the effect of variation in sequencing depth using the function rrarefy in vegan
有一步是按OTU丰度排序,所以必须抽平
如果按照otu的百分含量呢
按OTU丰度排序
按照OTU百分含量排序
如果不抽平,由于某个样本序列数够大会引入稀有OTU,而其他的样本则没有这个稀有OTU,从有无上判断,这会增大样本间的差距吗?如果基于百分比是否会好些?
拆分OTU表分组抽平